Metaphase and Anaphase
Upon entry into anaphase, the mitotic spindle reorganizes dramatically: kinetochore fibers attached to chromosomes shorten, bringing chromatids toward the poles. The astral microtubules, which radiate from each spindle pole, elongate until they reach the cell surface. Meanwhile, a bundle of antiparallel microtubules, called the ‘central spindle,’ remains at the midpoint between the two poles. The central spindle and astral microtubules collaborate to position the division plane during cytokinesis.